Journal: Cancer gene therapy

Article Title: Immunogenic cell death by oncolytic herpes simplex virus type 1 in squamous cell carcinoma cells.

doi: 10.1038/cgt.2016.8

Figure Lengend Snippet: Figure 3. Expression of calreticulin (CRT) on the cell surface. SCCVII cells were infected with RH2 at a multiplicity of infection (MOI) of 10, incubated for 24 h, and fixed in paraformaldehyde. Cells were stained with an anti-CRT antibody, Alexa Fluor 633-conjugated wheat germ agglutinin (WGA), and (4',6-diamidino-2-phenylindole) (DAPI) and then analyzed under a confocal laser-scanning microscope. A representative result was presented. PBS, phosphate-buffered saline; SCC, squamous cell carcinoma.

Article Snippet: After washing, the cells were incubated with an FITC (fluorescein isothiocyanate)-conjugated goat polyclonal antibody diluted 1:100 and Alexa Fluor 633-conjugated wheat germ agglutinin (WGA) (Invitrogen, Carlsbad, CA, USA) for 30 min. After washing, coverslips were mounted onto microslides using a ProLong Gold Antifade Reagent with DAPI (4',6-diamidino-2-phenylindole) (CST Japan, Tokyo, Japan).

Techniques: Expressing, Infection, Incubation, Staining, Laser-Scanning Microscopy, Saline

Journal: PLoS ONE

Article Title: Deformation of the Outer Hair Cells and the Accumulation of Caveolin-2 in Connexin 26 Deficient Mice

doi: 10.1371/journal.pone.0141258

Figure Lengend Snippet: (A-F) Immunofluorescence staining for CAV2 in the organ of Corti around apical part of cochlea in 3-week-old Cx26 f/f P0Cre mice with GJB2 -associated deafness and in littermate controls. Whole-mount cochleae were fixed and immunolabeled with anti-CAV2 (red). Nuclei were counterstained with DAPI (blue). In contrast to the controls, notable accumulation of CAV2 was observed at the organ of Corti in Cx26 f/f P0Cre cochlea e . (G) shows the mean percentage of cells with accumulated CAV2 in control and Cx26 f/f P0Cre cochleae. There was a statistically significant difference between the control and Cx26 f/f P0Cre mice. Values represent the mean ± SE (n = 5 mice). ***P = 3.72 × 10 −5 , Student’s t -test.

Article Snippet: Immunofluorescence staining with antibodies against CAV2 (mouse IgG; BD) and Prestin (goat IgG; Santa Cruz) along with DAPI (Vector Laboratories) was performed on whole-mount preparations of the carefully resected organ of Corti or cochlear cryosections (7 μm).

Techniques: Immunofluorescence, Staining, Immunolabeling

Journal: PLoS ONE

Article Title: Deformation of the Outer Hair Cells and the Accumulation of Caveolin-2 in Connexin 26 Deficient Mice

doi: 10.1371/journal.pone.0141258

Figure Lengend Snippet: Accumulation of CAV2 was observed in OHCs around apical part of cochlea and colocalized with prestin in 3-week-old control and Cx26 f/f P0Cre mice. z -stacks of images were collected at 0.5-μm intervals (B, C, E, F), and the images of OHCs were reconstructed with graphics software (A, D). Double labeling for CAV2 and prestin revealed that OHCs of Cx26 f/f P0Cre mice had an altered, hourglass-like shape and that CAV2 accumulated near the basolateral plasma membranes. In contrast, CAV2 accumulation was mainly observed surrounding the shrunken site of OHCs in Cx26 f/f P0Cre mice (arrowheads in D). OHCs, outer hair cells.

Article Snippet: Immunofluorescence staining with antibodies against CAV2 (mouse IgG; BD) and Prestin (goat IgG; Santa Cruz) along with DAPI (Vector Laboratories) was performed on whole-mount preparations of the carefully resected organ of Corti or cochlear cryosections (7 μm).

Techniques: Software, Labeling

Journal: PLoS ONE

Article Title: Deformation of the Outer Hair Cells and the Accumulation of Caveolin-2 in Connexin 26 Deficient Mice

doi: 10.1371/journal.pone.0141258

Figure Lengend Snippet: Cryosections of the organ of Corti around apical part of cochlea were immunolabeled with anti-CAV2 (red). Nuclei were counterstained with DAPI (blue). In contrast to the controls, the TC (dotted line with an arrow) was closed, and notable accumulation of CAVs was observed in OHCs and supporting cells. In particular, such accumulation was observed in cells surrounding the closed TC in Cx26 f/f P0Cre cochleae. Scare bars, 10 μm. IHC, inner hair cell; OHCs, outer hair cells; TC, tunnel of Corti.

Article Snippet: Immunofluorescence staining with antibodies against CAV2 (mouse IgG; BD) and Prestin (goat IgG; Santa Cruz) along with DAPI (Vector Laboratories) was performed on whole-mount preparations of the carefully resected organ of Corti or cochlear cryosections (7 μm).

Techniques: Immunolabeling

Journal: PLoS ONE

Article Title: Deformation of the Outer Hair Cells and the Accumulation of Caveolin-2 in Connexin 26 Deficient Mice

doi: 10.1371/journal.pone.0141258

Figure Lengend Snippet: (A) Western blotting revealed an increase in CAV2 level in Cx26 f/f P0Cre mice at postnatal day 21. (B) CAV level was normalized to that of β-actin and is expressed as relative to the amount present in each littermate control. Values represent the mean ± SE (n = 6 for control, n = 5 for Cx26 f/f P0Cre). **P = 2.5×10 −3 for CAV2, Student’s t -test.

Article Snippet: Immunofluorescence staining with antibodies against CAV2 (mouse IgG; BD) and Prestin (goat IgG; Santa Cruz) along with DAPI (Vector Laboratories) was performed on whole-mount preparations of the carefully resected organ of Corti or cochlear cryosections (7 μm).

Techniques: Western Blot